Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.
Identifieur interne : 001660 ( Main/Exploration ); précédent : 001659; suivant : 001661Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.
Auteurs : Mohan Babu [Canada] ; J Javier Díaz-Mejía ; James Vlasblom ; Alla Gagarinova ; Sadhna Phanse ; Chris Graham ; Fouad Yousif ; Huiming Ding ; Xuejian Xiong ; Anaies Nazarians-Armavil ; Md Alamgir ; Mehrab Ali ; Oxana Pogoutse ; Asaf Pe'Er ; Roland Arnold ; Magali Michaut ; John Parkinson ; Ashkan Golshani ; Chris Whitfield ; Shoshana J. Wodak ; Gabriel Moreno-Hagelsieb ; Jack F. Greenblatt ; Andrew EmiliSource :
- PLoS genetics [ 1553-7404 ] ; 2011.
English descriptors
- KwdEn :
- Cell Membrane (genetics), Culture Media, Drug Resistance (genetics), Epistasis, Genetic (genetics), Escherichia coli (genetics), Escherichia coli (growth & development), Escherichia coli (metabolism), Gene Expression Regulation, Bacterial, Gene-Environment Interaction, Membrane Proteins (genetics), Membrane Proteins (metabolism), Metabolic Networks and Pathways (genetics), Microscopy, Electron, Microtubule-Associated Proteins (genetics), Microtubule-Associated Proteins (metabolism), Molecular Sequence Annotation, Oligonucleotide Array Sequence Analysis.
- MESH :
- chemical , genetics : Membrane Proteins, Microtubule-Associated Proteins.
- chemical , metabolism : Membrane Proteins, Microtubule-Associated Proteins.
- chemical : Culture Media.
- genetics : Cell Membrane, Drug Resistance, Epistasis, Genetic, Escherichia coli, Metabolic Networks and Pathways.
- growth & development : Escherichia coli.
- metabolism : Escherichia coli.
- Gene Expression Regulation, Bacterial, Gene-Environment Interaction, Microscopy, Electron, Molecular Sequence Annotation, Oligonucleotide Array Sequence Analysis.
Abstract
As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target.
DOI: 10.1371/journal.pgen.1002377
PubMed: 22125496
Affiliations:
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Wodak</name></author><author><name sortKey="Moreno Hagelsieb, Gabriel" sort="Moreno Hagelsieb, Gabriel" uniqKey="Moreno Hagelsieb G" first="Gabriel" last="Moreno-Hagelsieb">Gabriel Moreno-Hagelsieb</name></author><author><name sortKey="Greenblatt, Jack F" sort="Greenblatt, Jack F" uniqKey="Greenblatt J" first="Jack F" last="Greenblatt">Jack F. Greenblatt</name></author><author><name sortKey="Emili, Andrew" sort="Emili, Andrew" uniqKey="Emili A" first="Andrew" last="Emili">Andrew Emili</name></author></analytic><series><title level="j">PLoS genetics</title><idno type="eISSN">1553-7404</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Membrane (genetics)</term><term>Culture Media</term><term>Drug Resistance (genetics)</term><term>Epistasis, Genetic (genetics)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (growth & development)</term><term>Escherichia coli (metabolism)</term><term>Gene Expression Regulation, Bacterial</term><term>Gene-Environment Interaction</term><term>Membrane Proteins (genetics)</term><term>Membrane Proteins (metabolism)</term><term>Metabolic Networks and Pathways (genetics)</term><term>Microscopy, Electron</term><term>Microtubule-Associated Proteins (genetics)</term><term>Microtubule-Associated Proteins (metabolism)</term><term>Molecular Sequence Annotation</term><term>Oligonucleotide Array Sequence Analysis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Proteins</term><term>Microtubule-Associated Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Proteins</term><term>Microtubule-Associated Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Membrane</term><term>Drug Resistance</term><term>Epistasis, Genetic</term><term>Escherichia coli</term><term>Metabolic Networks and Pathways</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Bacterial</term><term>Gene-Environment Interaction</term><term>Microscopy, Electron</term><term>Molecular Sequence Annotation</term><term>Oligonucleotide Array Sequence Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><noCountry><name sortKey="Alamgir, Md" sort="Alamgir, Md" uniqKey="Alamgir M" first="Md" last="Alamgir">Md Alamgir</name><name sortKey="Ali, Mehrab" sort="Ali, Mehrab" uniqKey="Ali M" first="Mehrab" last="Ali">Mehrab Ali</name><name sortKey="Arnold, Roland" sort="Arnold, Roland" uniqKey="Arnold R" first="Roland" last="Arnold">Roland Arnold</name><name sortKey="Diaz Mejia, J Javier" sort="Diaz Mejia, J Javier" uniqKey="Diaz Mejia J" first="J Javier" last="Díaz-Mejía">J Javier Díaz-Mejía</name><name sortKey="Ding, Huiming" sort="Ding, Huiming" uniqKey="Ding H" first="Huiming" last="Ding">Huiming Ding</name><name sortKey="Emili, Andrew" sort="Emili, Andrew" uniqKey="Emili A" first="Andrew" last="Emili">Andrew Emili</name><name sortKey="Gagarinova, Alla" sort="Gagarinova, Alla" uniqKey="Gagarinova A" first="Alla" last="Gagarinova">Alla Gagarinova</name><name sortKey="Golshani, Ashkan" sort="Golshani, Ashkan" uniqKey="Golshani A" first="Ashkan" last="Golshani">Ashkan Golshani</name><name sortKey="Graham, Chris" sort="Graham, Chris" uniqKey="Graham C" first="Chris" last="Graham">Chris Graham</name><name sortKey="Greenblatt, Jack F" sort="Greenblatt, Jack F" uniqKey="Greenblatt J" first="Jack F" last="Greenblatt">Jack F. Greenblatt</name><name sortKey="Michaut, Magali" sort="Michaut, Magali" uniqKey="Michaut M" first="Magali" last="Michaut">Magali Michaut</name><name sortKey="Moreno Hagelsieb, Gabriel" sort="Moreno Hagelsieb, Gabriel" uniqKey="Moreno Hagelsieb G" first="Gabriel" last="Moreno-Hagelsieb">Gabriel Moreno-Hagelsieb</name><name sortKey="Nazarians Armavil, Anaies" sort="Nazarians Armavil, Anaies" uniqKey="Nazarians Armavil A" first="Anaies" last="Nazarians-Armavil">Anaies Nazarians-Armavil</name><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><name sortKey="Pe Er, Asaf" sort="Pe Er, Asaf" uniqKey="Pe Er A" first="Asaf" last="Pe'Er">Asaf Pe'Er</name><name sortKey="Phanse, Sadhna" sort="Phanse, Sadhna" uniqKey="Phanse S" first="Sadhna" last="Phanse">Sadhna Phanse</name><name sortKey="Pogoutse, Oxana" sort="Pogoutse, Oxana" uniqKey="Pogoutse O" first="Oxana" last="Pogoutse">Oxana Pogoutse</name><name sortKey="Vlasblom, James" sort="Vlasblom, James" uniqKey="Vlasblom J" first="James" last="Vlasblom">James Vlasblom</name><name sortKey="Whitfield, Chris" sort="Whitfield, Chris" uniqKey="Whitfield C" first="Chris" last="Whitfield">Chris Whitfield</name><name sortKey="Wodak, Shoshana J" sort="Wodak, Shoshana J" uniqKey="Wodak S" first="Shoshana J" last="Wodak">Shoshana J. Wodak</name><name sortKey="Xiong, Xuejian" sort="Xiong, Xuejian" uniqKey="Xiong X" first="Xuejian" last="Xiong">Xuejian Xiong</name><name sortKey="Yousif, Fouad" sort="Yousif, Fouad" uniqKey="Yousif F" first="Fouad" last="Yousif">Fouad Yousif</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Babu, Mohan" sort="Babu, Mohan" uniqKey="Babu M" first="Mohan" last="Babu">Mohan Babu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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